'Aai No. 1' is a slapstick comedy that highlights the heroism of a brave mother. Jayaram Londhe (Sanjay Narvekar) is an inexperienced policeman. Because of his immature approach, Jayaram's colleagues make fun of him. Unable to tolerate her son's insults, his mother, Mogarabai (Rasika Joshi) decides to help him secretly. Whenever Jayaram needs to go for an encounter, Mogarabai reaches there before him and fights the hooligans. Jayaram is praised for the achievement which he has not achieved. Soon, Jayaram gets promoted and his responsibilities increase. Will Mogarabai be able to support Jayaram for long? After an extent, Jayaram will have to fight for his life. Will Jayaram ever learn a lesson from his shrewd and courageous 'Aai No. 1'?