Ines Sastre was born in Valladolid (Spain). Her career began age 12, when she was selected to appear in a fast-food commercial. This appearance led her to be noticed by the producer Carlos Saura, who chose her to play in "El Dorado" with Lambert Wilson (1987). The film made the official selection of the Cannes Film Festival in 1988, this marked the beginning of her international acting career.
Ines also achieved international notoriety as a top model and succeeded Isabella Rossellini as the face of Lancome's emblematic Tresor fragrance. In addition to her acting and modeling career, Ines also completed studies in French modern literature at La Sorbonne University.
Ines Sastre returned to cinema in Italy to play for two of the most famous Italian directors; Michelangelo Antonioni in "Beyond the Clouds" (1996) together with John Malkovitch, Sophie Marceau and Fanny Ardant, and Pupi Avati in "The Best Man" (1998).
Ines Sastre also made a noticeable return to French cinema starring as Epona, the wife of Vercingetorix, in "Druids" (2001) by Jacques Dorfmann, alongside Max von Sidow and Christophe Lambert. That same year Ines shone alongside Guillaume Canet and Andre Dussolier as a manipulative courtesan in "Vidocq" by Pitof, were she was reunited with the infamous Gerard Depardieu that she had previously starred with in the TV mini-series "The Count of Monte-Cristo" by Josee Dayan.
Ines Sastre has also starred in two American productions. She made an appearance in "Sabrina" (1996) by Sidney Pollack, with Harrison Ford and was outstanding in the melodramatic "The Lost City" by and with Andy Garcia, Bill Murray and Dustin Hoffman.