Kiron Kher was born in Mumbai on June 14, 1955, to Sikh parents, and brought up in Chandigarh, Punjab, where she did her schooling and later college from Punjab University, Chandigarh. At school she was an avid Badminton player along with her sister, Arjuna awardee Kanwal Thakar Singh, their mother Diljit Singh was also active in sports and dramatics in in her college days in pre-partition Punjab . Kiron also had a brother, painter Amandeep Singh, who passed away in 2003.
Kiron Kher made her film debut in 1983 in the Punjabi feature film Aasra Pyar Da and received rave reviews for her performance. After her divorce to Gautam Berry ,in order to raise her son Sikander ...she worked for Anupam Kher as a costume designer .She suggested the title for his movie Dilwale Dulhania Le Jayenge.
she first met Anupam Kher at Department of Indian Theatre in Chandigarh in 1974, whom she married later. Anupam Kher has adopted Sikandar legally.
In 2003, she changed her name from "Kiron" to "Kirron" due to beliefs in numerology.