Niharika Khan was born in Jamshedpur. She studied Human Resources in the US and then worked in public relations for some time. She also did a corporate job in Tanishq and then with Disney, before taking up fashion jewellery full time. She launched her multi-brand store, Purple Porcupine in Bandra, Mumbai in 2003.
Her friend, Sameer Sharma introduced her to Sudhir Mishra, who asked her to do styling for his period film, Khoya Khoya Chand.
After this, there was no looking back and she got Rock On!! and also Karthik Calling Karthik. She became famous with Band Baaja Baaraat and followed it with Delhi Belly. She also did styling for Vidya Balan in The Dirty Picture. She is married to actor Ayub Khan and her brother is famous designer, Arjun Khanna.