Sadhana was born in 1924 at Patna. Her father was Satyasundar Basu and mother was Maitree Bose respectively. She metric examination from Bankipur Girls school of Patna. She was a IA from Bethune College and BA from Victoria college.
She made her film debut in “Diner Par Din” directed by Jyotirmoy Roy in 1949. She was basically a stage actress but she has acted in atleast 50 Bengali Films including “Chhele Kar”, “Ganga”, “Swapna Neaey”, “Bar Badhu”, “Tahader Katha”, etc. She has acted under the direction of Ritwik Ghatak and Rajen Tarafdar and Buddhadeb Dasgupta. She knew dancing and singing very well. She was also associated with Indian People theatre. She has also acted in Hindi Films and Radio Drama. She married twice. Her first husband was Kartik RoyChoudhury. Then she got married with actor director Sekhar Chattopadhay. They had one daughter. Sadhana has two sisters and they both were actress.