Sayali Bhagat was born in Nashik, Maharashtra in a traditional family. She studied at Fravashi Academy, Nasik, and completed her graduation in BMS (Bachelor of Management Studies) from Alkesh Dinesh Mody Institute of Finance and Management Studies (Mumbai University campus, Kalina, Santacruz).
Like the other beauty contest winners, she too entered the modeling industry, followed by Bollywood. Her initial modeling assignments were for Dentzz, SNDT College show and Swarovski gems fashion show.
The Train (released on 8 July 2007) was her debut Hindi film, co-starring Emraan Hashmi and Geeta Basra. She also appeared as a journalist from Singapore, who pretends to interview Indian cricketer Rahul Dravid on the show MTV Bakra. In 2009, she was seen in the Hindi movie, Paying Guests, opposite Javed Jaffrey then with Govinda in Naughty@40. She also appeared in a music video for the popular Punjabi song "Aish Karo" by A.S. Kang.